Aktivitas Fungisida Ekstrak Daun Sirih (Piper Betle L.) Kultivar Beleng terhadap Jamur Fusarium Oxysporum f.sp. Vanillae Penyebab Penyakit Busuk Batang pada Vanili

Fungicidal Activity of Betel Leaves (Piper Betle L.) of Beleng Cultivar on Fusarium Oxysporum f.sp. Vanillae Causes Stem Rot in Vanilla

DOI:

https://doi.org/10.59672/emasains.v8i1.269

Keywords:

fungicidal activity, betle leaf, fusarium oxysporum f. sp.vanillae.

Abstract

This research was done in order to know inhibitory activity of beatle leaf the cultivar of Beleng  extract and its mechanism against the growth of F. oxysporum f. sp. vanillae, that causal agent of steam disease on vanilla. Many effort have been done to control the disease such as the use of synthetic fungicide. In order to find other alternatives for controlling the disease, Beleng leaf were studied for their bioactivity against F. oxysporum f. sp. vanillae. The result of the study indicated that the crude extract of Beleng leaf at concentration 0.05%; 0.1%; 0.15%; 0.2%; 0.25%; 0.3% and 0.35% was able to inhibit the radial growth of F. oxysporum f.sp. vanillae on Potato Dextrose Agar (PDA) medium. The variation of crude extract concentration was found to influence the fungal growth on vanilla stem cutings on PD Broth medium. In addition, the crude extract of Beleng leaf was able to inhibit the spore germination and spore formation of F. oxysporum f. sp. vanillae on PD Broth medium. The fractination of Beleng leaf crude extract with Column Chromatography and Thin Layer Chromatography produced 15 fractions of compound, the fraction number VI which is made of three compounds with Rf value : 0.35; 0.55 and 0.78 efectively inhibited the growth of  F. oxysporum f. sp. vanillae on PDA medium.

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References

Agrios, G.N. (1988). Plant Pathology. San Diego, California: Academic Press. Inc.803p.
Borges, A. A, A. Borges-Perrez, M. Fernandez-Falcon. (2004). Induced Resistance to Fusarial Wilt of Banana by Menadione Sodium Bisulphite Treatments. Crop Protection, 23: 1245-1247.
CMI. (1978). Descriptions of Pathogenic Fungi and Bacteria. England: Ferry Lane, Kew Surrey. 419 p.
Heyne, K. (1987). Tumbuhan Berguna Indonesia Jilid II. Jakarta: Yayasan Sarana Wana Jaya. 631 h.
Kardinan, A. (2005). Pestisida Nabati Ramuan & Aplikasi. Jakarta: Penebar Swadaya. 88 h.
Moeljanto, R. D. dan Mulyono. (2003). Khasiat & Manfaat Daun Sirih Obat Mujarab dari Masa ke Masa. Jakarta : Agromedia Pustaka. 77 h.
Robinson, T. (1995). Kandungan Organik Tumbuhan Tinggi. Bandung: ITB. 367 h.
Ruhnayat, A. (2004). Bertanam Vanili si Emas Hijau Nan Wangi. Jakarta: Agromedia Pustaka. 51 h.

Rustini, N. L. (2004). Aktivitas Fungisida Ekstrak Rimpang Dringo (Acorus calamus L.) terhadap Jamur Botryodiplodia Theobromae Penyebab Penyakit Busuk Buah Pisang. Tesis. Denpasar : Universitas Udayana. 50 h.
Salleh, B. (1989). Perkembangan Mutakhir Penelitian Fusarium di Kawasan Tropika, Naskah Lengkap Kongres Nasional X dan Seminar Ilmiah Perhimpunan Fitopatologi Indonesia Denpasar. Denpasar 14 – 16 Nopember 1989: 11 – 18.
Semangun, H. (2000). Penyakit-Penyakit Tanaman Hortikultura Di Indonesia. Yogyakarta: Gajah Mada University Press. 850 h.
Semangun, H. (2001). Pengantar Ilmu Penyakit Tumbuhan. Yogyakarta: Gajah Mada University Press. 754 h.
Suharyon dan A.Rozak AR. (1996). Teknik Budidaya Panili (Vanilla Planifolia Andrew) di Antara Tanaman Kelapa Dalam Mana. Departemen Pertanian.
29 h.
Suprapta, D. N. (2001). Senyawa Antimikroba dan Pertahanan Tumbuhan Terhadap Infeksi Jamur. Agritrop, 20: 52-55.
Suprapta, D. N., I G. A. N. A. Suwari, N. Arya and K. Ohsawa. (2002). Pometia Pinnata Leaves Extract to Control Late Blight Disease of Tomato. Journal of ISSAAS, 8: 31-36.
Suprapta, D. N., I B.G. Darmayasa, N. Arya, I G. R. M. Temaja and K. Ohsawa. (2003). Bacterial Activity of Spaeranthus Indicus Extract Against Ralstonia Solanacearum in Tomato. Journal of ISSAAS, 9: 69-74.
Suprapta, D.N., M. Subrata, K. Siadi, I.G.A. Rai, F. Tunnisa and K. Ohsawa. (2006). Fungicidal Activity of Extract of Several Piperaceae Plants Against Fusarium Oxysporum f.sp. Vanillae. Academic Frontier Research Centre, Tokyo University of Agriculture. 44-52.
Yanti, R., Suyitno dan E. Harmayani. (2000). Identifikasi Komponen Ekstrak Sirih (Piper Betle Linn.) dari Beberapa Pelarut dan Pemanfaatannya Untuk Pengawetan Ikan. Agrosains, 13: 239-250.

Published

2019-05-06

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Articles